更新時間:2016-06-21
人小細胞肺癌細胞;NCI-H446,開始培養(yǎng)保持細胞濃度在2×10 exp5個細胞/ ml和1×10 exp5和1×10 exp6個細胞/ ml之間。只用于實驗室研究。不能被用于在臨床檢測和有關(guān)于人身安全的實驗。
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人小細胞肺癌細胞;NCI-H446
形態(tài)特性 上皮樣
生長特性 貼壁/懸浮生長,混合
特征特性人小細胞肺癌細胞;NCI-H446該細胞是1982年由Carney D和Gazdar AF等從一位小細胞肺癌患者的胸腔積液中建立的。細胞的原始形態(tài)并不具有小細胞肺癌特征。這個細胞株是小細胞肺癌的生化和形態(tài)學上的變種,表達神經(jīng)元*的烯醇酶和腦型肌酸激酶同工酶;左旋多巴脫羧酶、蠶素、抗利尿激素、催產(chǎn)素或胃泌激素釋放肽未達到可檢測水平。與正常細胞相比,該細胞c-myc DNA序列擴增約20倍,RNA增加15倍。zui初傳代培養(yǎng)基用含有5%FBS的RPMI1640,另外添加0.005 mg/ml胰島素、0.01 mg/ml轉(zhuǎn)鐵
培養(yǎng)條件 RPMI 1640 (w/o Hepes) 10%FBS
傳代方法 1:3~1:9傳代;每周換液2~3次。
傳代情況 C5
凍存條件 基礎培養(yǎng)基+5%DMSO+20%FBS
公司細胞廣泛用于國內(nèi)院校的細胞生物學研究,他們的很多論文都或多或少使用我們公司的細胞,作為實驗項目研究
白介素27w(IL27w)檢測試劑盒(化學發(fā)光免疫分析法) CLIA Kit for Interleukin 27 Working Designation (IL27w) X17630 96T Rattus norvegicus (Rat)
粒細胞巨噬細胞集落刺激因子(GMCSF)檢測試劑盒(化學發(fā)光免疫分析法) CLIA Kit for Colony Stimulating Factor 2, Granulocyte Macrophage (GMCSF) X17631 96T Canis familiaris; Canine (Dog)
粒細胞巨噬細胞集落刺激因子(GMCSF)檢測試劑盒(化學發(fā)光免疫分析法) CLIA Kit for Colony Stimulating Factor 2, Granulocyte Macrophage (GMCSF) X17632 96T Homo sapiens (Human)
粒細胞巨噬細胞集落刺激因子(GMCSF)檢測試劑盒(化學發(fā)光免疫分析法) CLIA Kit for Colony Stimulating Factor 2, Granulocyte Macrophage (GMCSF) X17633 96T Mus musculus (Mouse)
肝細胞生長因子(HGF)檢測試劑盒(化學發(fā)光免疫分析法) CLIA Kit for Hepatocyte Growth Factor (HGF) X17634 96T Bos taurus; Bovine (Cattle)
肝細胞生長因子(HGF)檢測試劑盒(化學發(fā)光免疫分析法) CLIA Kit for Hepatocyte Growth Factor (HGF) X17635 96T Canis familiaris; Canine (Dog)
肝細胞生長因子(HGF)檢測試劑盒(化學發(fā)光免疫分析法) CLIA Kit for Hepatocyte Growth Factor (HGF) X17636 96T Mus musculus (Mouse)
肝細胞生長因子(HGF)檢測試劑盒(化學發(fā)光免疫分析法) CLIA Kit for Hepatocyte Growth Factor (HGF) X17637 96T Rattus norvegicus (Rat)
肝細胞生長因子(HGF)檢測試劑盒(化學發(fā)光免疫分析法) CLIA Kit for Hepatocyte Growth Factor (HGF) X17638 96T Oryctolagus cuniculus (Rabbit)
干擾素γ(IFNγ)檢測試劑盒(化學發(fā)光免疫分析法) CLIA Kit for Interferon Gamma (IFNg) X17639 96T Bos taurus; Bovine (Cattle)
干擾素γ(IFNγ)檢測試劑盒(化學發(fā)光免疫分析法) CLIA Kit for Interferon Gamma (IFNg) X17640 96T Canis familiaris; Canine (Dog)
干擾素γ(IFNγ)檢測試劑盒(化學發(fā)光免疫分析法) CLIA Kit for Interferon Gamma (IFNg) X17641 96T Chicken (Gallus)
干擾素γ(IFNγ)檢測試劑盒(化學發(fā)光免疫分析法) CLIA Kit for Interferon Gamma (IFNg) X17642 96T Cavia (Guinea pig )
干擾素γ(IFNγ)檢測試劑盒(化學發(fā)光免疫分析法) CLIA Kit for Interferon Gamma (IFNg) X17643 96T Sus scrofa; Porcine (Pig)
干擾素γ(IFNγ)檢測試劑盒(化學發(fā)光免疫分析法) CLIA Kit for Interferon Gamma (IFNg) X17644 96T Oryctolagus cuniculus (Rabbit)
干擾素γ(IFNγ)檢測試劑盒(化學發(fā)光免疫分析法) CLIA Kit for Interferon Gamma (IFNg) X17645 96T Rhesus monkey (Simian)
粒細胞集落刺激因子(GCSF)檢測試劑盒(化學發(fā)光免疫分析法) CLIA Kit for Colony Stimulating Factor 3, Granulocyte (GCSF) X17646 96T Homo sapiens (Human)
粒細胞集落刺激因子(GCSF)檢測試劑盒(化學發(fā)光免疫分析法) CLIA Kit for Colony Stimulating Factor 3, Granulocyte (GCSF) X17647 96T Mus musculus (Mouse)
粒細胞集落刺激因子(GCSF)檢測試劑盒(化學發(fā)光免疫分析法) CLIA Kit for Colony Stimulating Factor 3, Granulocyte (GCSF) X17648 96T Rattus norvegicus (Rat)
膠質(zhì)細胞系來源神經(jīng)營養(yǎng)因子(GDNF)檢測試劑盒(化學發(fā)光免疫分析法) CLIA Kit for Glial Cell Line Derived Neurotrophic Factor (GDNF) X17649 96T Mus musculus (Mouse)
膠質(zhì)細胞系來源神經(jīng)營養(yǎng)因子(GDNF)檢測試劑盒(化學發(fā)光免疫分析法) CLIA Kit for Glial Cell Line Derived Neurotrophic Factor (GDNF) X17650 96T Rattus norvegicus (Rat)
核糖核酸酶T2(RNASET2)檢測試劑盒(化學發(fā)光免疫分析法) CLIA Kit for Ribonuclease T2 (RNASET2) X17651 96T Homo sapiens (Human)
核糖核酸酶T2(RNASET2)檢測試劑盒(化學發(fā)光免疫分析法) CLIA Kit for Ribonuclease T2 (RNASET2) X17652 96T Mus musculus (Mouse)
核糖核酸酶T2(RNASET2)檢測試劑盒(化學發(fā)光免疫分析法) CLIA Kit for Ribonuclease T2 (RNASET2) X17653 96T Rattus norvegicus (Rat)
組織金屬蛋白酶抑制因子3(TIMP3)檢測試劑盒(化學發(fā)光免疫分析法) CLIA Kit for Tissue Inhibitors Of Metalloproteinase 3 (TIMP3) X17654 96T Bos taurus; Bovine (Cattle)
組織金屬蛋白酶抑制因子3(TIMP3)檢測試劑盒(化學發(fā)光免疫分析法) CLIA Kit for Tissue Inhibitors Of Metalloproteinase 3 (TIMP3) X17655 96T Capra hircus; Caprine (Goat)
組織金屬蛋白酶抑制因子3(TIMP3)檢測試劑盒(化學發(fā)光免疫分析法) CLIA Kit for Tissue Inhibitors Of Metalloproteinase 3 (TIMP3) X17656 96T Equus caballus; Equine (Horse)
組織金屬蛋白酶抑制因子3(TIMP3)檢測試劑盒(化學發(fā)光免疫分析法) CLIA Kit for Tissue Inhibitors Of Metalloproteinase 3 (TIMP3) X17657 96T Chicken (Gallus)
組織金屬蛋白酶抑制因子3(TIMP3)檢測試劑盒(化學發(fā)光免疫分析法) CLIA Kit for Tissue Inhibitors Of Metalloproteinase 3 (TIMP3) X17658 96T Mus musculus (Mouse)
組織金屬蛋白酶抑制因子3(TIMP3)檢測試劑盒(化學發(fā)光免疫分析法) CLIA Kit for Tissue Inhibitors Of Metalloproteinase 3 (TIMP3) X17659 96T Sus scrofa; Porcine (Pig)
組織金屬蛋白酶抑制因子3(TIMP3)檢測試劑盒(化學發(fā)光免疫分析法) CLIA Kit for Tissue Inhibitors Of Metalloproteinase 3 (TIMP3) X17660 96T Rattus norvegicus (Rat)
組織金屬蛋白酶抑制因子3(TIMP3)檢測試劑盒(化學發(fā)光免疫分析法) CLIA Kit for Tissue Inhibitors Of Metalloproteinase 3 (TIMP3) X17661 96T Oryctolagus cuniculus (Rabbit)
組織金屬蛋白酶抑制因子3(TIMP3)檢測試劑盒(化學發(fā)光免疫分析法) CLIA Kit for Tissue Inhibitors Of Metalloproteinase 3 (TIMP3) X17662 96T Rhesus monkey (Simian)
Rho鳥嘌呤核苷酸交換因子25(ARHGEF25)檢測試劑盒(化學發(fā)光免疫分析法) CLIA Kit for Rho Guanine Nucleotide Exchange Factor 25 (ARHGEF25) X17663 96T Homo sapiens (Human)
Rho鳥嘌呤核苷酸交換因子25(ARHGEF25)檢測試劑盒(化學發(fā)光免疫分析法) CLIA Kit for Rho Guanine Nucleotide Exchange Factor 25 (ARHGEF25) X17664 96T Mus musculus (Mouse)
Rho鳥嘌呤核苷酸交換因子25(ARHGEF25)檢測試劑盒(化學發(fā)光免疫分析法) CLIA Kit for Rho Guanine Nucleotide Exchange Factor 25 (ARHGEF25) X17665 96T Rattus norvegicus (Rat)
干擾素調(diào)節(jié)因子2結(jié)合蛋白1(IRF2BP1)檢測試劑盒(化學發(fā)光免疫分析法) CLIA Kit for Interferon Regulatory Factor 2 Binding Protein 1(IRF2BP1) X17666 96T Homo sapiens (Human)
干擾素調(diào)節(jié)因子2結(jié)合蛋白1(IRF2BP1)檢測試劑盒(化學發(fā)光免疫分析法) CLIA Kit for Interferon Regulatory Factor 2 Binding Protein 1(IRF2BP1) X17667 96T Mus musculus (Mouse)
干擾素調(diào)節(jié)因子2結(jié)合蛋白1(IRF2BP1)檢測試劑盒(化學發(fā)光免疫分析法) CLIA Kit for Interferon Regulatory Factor 2 Binding Protein 1(IRF2BP1) X17668 96T Rattus norvegicus (Rat)
干擾素調(diào)節(jié)因子2結(jié)合蛋白2(IRF2BP2)檢測試劑盒(化學發(fā)光免疫分析法) CLIA Kit for Interferon Regulatory Factor 2 Binding Protein 2(IRF2BP2) X17669 96T Homo sapiens (Human)
干擾素調(diào)節(jié)因子2結(jié)合蛋白2(IRF2BP2)檢測試劑盒(化學發(fā)光免疫分析法) CLIA Kit for Interferon Regulatory Factor 2 Binding Protein 2(IRF2BP2) X17670 96T Mus musculus (Mouse)
成纖維細胞生長因子結(jié)合蛋白3(FGFBP3)檢測試劑盒(化學發(fā)光免疫分析法) CLIA Kit for Fibroblast Growth Factor Binding Protein 3 (FGFBP3) X17671 96T Homo sapiens (Human)
成纖維細胞生長因子結(jié)合蛋白3(FGFBP3)檢測試劑盒(化學發(fā)光免疫分析法) CLIA Kit for Fibroblast Growth Factor Binding Protein 3 (FGFBP3) X17672 96T Mus musculus (Mouse)
成纖維細胞生長因子結(jié)合蛋白3(FGFBP3)檢測試劑盒(化學發(fā)光免疫分析法) CLIA Kit for Fibroblast Growth Factor Binding Protein 3 (FGFBP3) X17673 96T Rattus norvegicus (Rat)
血管內(nèi)皮生長因子145(VEGF145)檢測試劑盒(化學發(fā)光免疫分析法) CLIA Kit for Vascular Endothelial Growth Factor 145 (VEGF145) X17674 96T Homo sapiens (Human)
肝癌衍生生長因子樣蛋白1(HDGFL1)檢測試劑盒(化學發(fā)光免疫分析法) CLIA Kit for Hepatoma Derived Growth Factor Like Protein 1(HDGFL1) X17675 96T Homo sapiens (Human)
肝癌衍生生長因子樣蛋白1(HDGFL1)檢測試劑盒(化學發(fā)光免疫分析法) CLIA Kit for Hepatoma Derived Growth Factor Like Protein 1(HDGFL1) X17676 96T Mus musculus (Mouse)
肝癌衍生生長因子樣蛋白1(HDGFL1)檢測試劑盒(化學發(fā)光免疫分析法) CLIA Kit for Hepatoma Derived Growth Factor Like Protein 1(HDGFL1) X17677 96T Rattus norvegicus (Rat)
神經(jīng)元衍生神經(jīng)營養(yǎng)因子(NDNF)檢測試劑盒(化學發(fā)光免疫分析法) CLIA Kit for Neuron Derived Neurotrophic Factor (NDNF) X17678 96T Homo sapiens (Human)
錨定蛋白重復域蛋白50(ANKRD50)檢測試劑盒(化學發(fā)光免疫分析法) CLIA Kit for Ankyrin Repeat Domain Protein 50 (ANKRD50) X17679 96T Homo sapiens (Human)
錨定蛋白重復域蛋白50(ANKRD50)檢測試劑盒(化學發(fā)光免疫分析法) CLIA Kit for Ankyrin Repeat Domain Protein 50 (ANKRD50) X17680 96T Mus musculus (Mouse)
錨定蛋白重復域蛋白50(ANKRD50)檢測試劑盒(化學發(fā)光免疫分析法) CLIA Kit for Ankyrin Repeat Domain Protein 50 (ANKRD50) X17681 96T Rattus norvegicus (Rat)
對氧磷酶1(PON1)檢測試劑盒(化學發(fā)光免疫分析法) CLIA Kit for Paraoxonase 1 (PON1) X17682 96T Homo sapiens (Human)
D-二聚體(D2D)檢測試劑盒(化學發(fā)光免疫分析法) CLIA Kit for D-Dimer (D2D) X17683 96T Mus musculus (Mouse)
載脂蛋白A1(APOA1)檢測試劑盒(化學發(fā)光免疫分析法) CLIA Kit for Apolipoprotein A1 (APOA1) X17684 96T Mus musculus (Mouse)
肌原纖蛋白1(FBN1)檢測試劑盒(化學發(fā)光免疫分析法) CLIA Kit for Fibrillin 1 (FBN1) X17685 96T Homo sapiens (Human)
粘蛋白5B(MUC5B)檢測試劑盒(化學發(fā)光免疫分析法) CLIA Kit for Mucin 5 Subtype B (MUC5B) X17686 96T Homo sapiens (Human)
Toll樣受體4(TLR4)檢測試劑盒(化學發(fā)光免疫分析法) CLIA Kit for Toll Like Receptor 4 (TLR4) X17687 96T Mus musculus (Mouse)
血清淀粉樣P物質(zhì)(SAP)檢測試劑盒(化學發(fā)光免疫分析法) CLIA Kit for Serum Amyloid P Component (SAP) X17688 96T Homo sapiens (Human)
巨噬細胞清道夫受體1(MSR1)檢測試劑盒(化學發(fā)光免疫分析法) CLIA Kit for Macrophage Scavenger Receptor 1 (MSR1) X17689 96T Homo sapiens (Human)
主要堿性蛋白(MBP)檢測試劑盒(化學發(fā)光免疫分析法) CLIA Kit for Major Basic Protein (MBP) X17690 96T Mus musculus (Mouse)
黑素瘤細胞黏附分子(MCAM)檢測試劑盒(化學發(fā)光免疫分析法) CLIA Kit for Melanoma Cell Adhesion Molecule (MCAM) X17691 96T Mus musculus (Mouse)
載脂蛋白A4(APOA4)檢測試劑盒(化學發(fā)光免疫分析法) CLIA Kit for Apolipoprotein A4 (APOA4) X17692 96T Homo sapiens (Human)
上皮型脂肪酸結(jié)合蛋白(FABP5)檢測試劑盒(化學發(fā)光免疫分析法) CLIA Kit for Fatty Acid Binding Protein 5, Epidermal (FABP5) X17693 96T Homo sapiens (Human)
整合素β6(ITGβ6)檢測試劑盒(化學發(fā)光免疫分析法) CLIA Kit for Integrin Beta 6 (ITGb6) X17694 96T Homo sapiens (Human)
膜聯(lián)蛋白A1(ANXA1)檢測試劑盒(化學發(fā)光免疫分析法) CLIA Kit for Annexin A1 (ANXA1) X17695 96T Homo sapiens (Human)
嘌呤能受體P2X7(P2RX7)檢測試劑盒(化學發(fā)光免疫分析法) CLIA Kit for Purinergic Receptor P2X, Ligand Gated Ion Channel 7 (P2RX7) X17696 96T Homo sapiens (Human)
絲聚蛋白(FLG)檢測試劑盒(化學發(fā)光免疫分析法) CLIA Kit for Filaggrin (FLG) X17697 96T Homo sapiens (Human)
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