更新時(shí)間:2016-06-20
人結(jié)直腸腺癌細(xì)胞;LS 174T [LS174T],開(kāi)始培養(yǎng)保持細(xì)胞濃度在2×10 exp5個(gè)細(xì)胞/ ml和1×10 exp5和1×10 exp6個(gè)細(xì)胞/ ml之間。只用于實(shí)驗(yàn)室研究。不能被用于在臨床檢測(cè)和有關(guān)于人身安全的實(shí)驗(yàn)。
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人結(jié)直腸腺癌細(xì)胞;LS 174T [LS174T]
形態(tài)特性 上皮樣
生長(zhǎng)特性 貼壁生長(zhǎng)
特征特性人結(jié)直腸腺癌細(xì)胞;LS 174T [LS174T]該細(xì)胞是LS 180結(jié)腸腺癌細(xì)胞株的胰蛋白酶化變種。它比親本更易傳代,像LS 180一樣生成大量的癌胚抗原(CEA)。電鏡研究表明,它有豐富的微絲和胞漿黏液素液泡;血清學(xué)檢測(cè)直腸抗原3陽(yáng)性;p53抗原表達(dá)陰性,但mRNA表達(dá)陽(yáng)性;角蛋白陽(yáng)性;癌基因c-myc、N-myc、H-ras、N-ras、Myb和fos的表達(dá)呈陽(yáng)性;癌基因k-ras和sis的表達(dá)未做檢測(cè);可產(chǎn)生IL-10、IL-6和黏蛋白。
培養(yǎng)條件 MEM-EBSS: Minimum Essential Medium (MEM Eagles with Earle's Balanced Salts) 10%FBS
傳代方法 1:2~1:4傳代;每周換液2~3次。
傳代情況 P18
凍存條件 基礎(chǔ)培養(yǎng)基+5%DMSO+20%FBS
公司細(xì)胞廣泛用于國(guó)內(nèi)院校的細(xì)胞生物學(xué)研究,他們的很多論文都或多或少使用我們公司的細(xì)胞,作為實(shí)驗(yàn)項(xiàng)目研究
白花前胡素E Praeruptorin E人結(jié)直腸腺癌細(xì)胞;LS 174T [LS174T]X27179 "≥98% "
白樺脂酸 Betulinic acid X27180 "≥98% "
"白屈菜堿 " Chelidonine X27181 "≥98% "
白術(shù)內(nèi)酯 III Atractylenolide III X27182 "≥98% "
白術(shù)內(nèi)酯Ⅰ Atractylenolide I X27183 "≥98% "
"白頭翁素 " ANEMONIN X27184 "≥98% "
"白鮮堿 " Dictamnine X27185 "≥98% "
斑蝥素 Cantharidin X27186 ≥98%
"貝萼皂苷元 " Bayogenin X27187 "≥98% "
"扁塑藤素 " Pristimerin X27188 "≥98% "
扁蓄苷 Quercetin 3-O-alpha-L-arabinoside X27189 "≥98% "
表兒茶素沒(méi)食子酸酯 Epicatechin gallate X27190 "≥98% "
表沒(méi)食子兒茶素沒(méi)食子酸酯 EGCG X27191 "≥98% "
"檳榔堿 " Arecoline hydrobromide X27192 "≥98% "
補(bǔ)骨脂甲素 , 補(bǔ)骨脂二氫黃酮 Bavachin X27193 97%
補(bǔ)骨脂乙素,異補(bǔ)骨脂查爾酮 Isobavachalcone X27194 97%
蒼術(shù)苷A atractyloside A X27195 ≥97%
蒼術(shù)素 Atractylodin X27196 "≥97% "
藏紅花酸 Alpha-Crocetin X27197 "≥95% "
岑酮 Fraxinellone X27198 "≥98% "
柴胡皂苷C Saikosaponin C X27199 "≥97% "
"蟾毒靈 " Bufalin X27200 "≥98% "
蟾毒他靈 Bufotaline X27201 98%
"長(zhǎng)春胺 " Vincamine X27202 "≥95% "
"長(zhǎng)春地辛 " Vindesine X27203 "≥95% "
長(zhǎng)春花堿 Vinblastine X27204 "≥98% "
"長(zhǎng)春堿 " Vinblastine X27205 "≥95% "
長(zhǎng)春西汀 Vinpocetine X27206 ≥98%
長(zhǎng)春新堿 Vincristine X27207 "≥92% "
長(zhǎng)春質(zhì)堿 Catharanthine X27208 ≥95%
朝藿定A Epimedin A X27209 "≥98% "
朝藿定B Epimedin B X27210 "≥98% "
朝藿定C Epimedin C X27211 "≥98% "
翅子羅漢果I Siamenoside I X27212 "≥98% "
川陳皮素 Nobiletin X27213 "≥98% "
川芎嗪 Ligustrazine X27214 "≥98% "
"穿心蓮內(nèi)酯,穿心蓮乙素 " Andrographolide X27215 "≥98% "
穿心蓮乙素 Andrographolide X27216 98%
"次百部堿 " Stemonidine X27217 ≥98%
刺五加苷B Eleutheroside B X27218 95%
"刺五加苷D " Eleutheroside D X27219 "≥98% "
"刺五加皂苷B " acanthopanax senticosides B X27220 "≥95% "
大豆皂苷AA Soyasaponin Aa X27221 "≥98% "
大豆皂苷BB Soyasaponin Bb X27222 "≥97% "
"大薊苷 " Pectolinarin X27223 "≥98% "
"大葉茜草素 " Mollugin X27224 "≥98% "
丹參酚酸A Salvianolic acid A X27225 ≥96%
丹參酮Ⅰ Tanshinone I X27226 "≥99% "
丹參酮ⅡA Tanshinone II A X27227 "≥98% "
丹酚酸C Salvianolic acid C X27228 98%
"靛玉紅 " Indirubin X27229 "≥98% "
"丁香酚 " Eugenol X27230 "≥98% "
丁香樹(shù)脂醇雙葡萄糖苷 Syringaresinol-di-O-glucoside X27231 95%
丁香酸/3,5-二甲氧基-4-羥基苯甲酸 Syringic acid人結(jié)直腸腺癌細(xì)胞;LS 174T [LS174T]X27232 98%
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